Optimisation of Conditions for the Affinity Chromatography of Human Enterokinase on Immobilised p-Aminobenzamidine. Improvement of the Preparative Procedure by Inclusion of Negative Affinity Chromatography with Glycylglycyl-aniline
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متن کاملPurification of porcine enterokinase by affinity chromatography.
A method is described for the purification of porcine enterokinase by affinity chromatography with p-aminobenzamidine as the ligand. Purification was completed by immunoadsorption with antisera raised to components binding non-biologically to the gel. The final enterokinase preparation was 2.3 times more active than the most active preparation previously described.
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1978
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1978.tb12436.x